ProDuSe: Process Duplex Sequence data

Christopher Rushton
Marco Albuquerque
Source Code:Github
License:GNU General Public License v3.0

ProDuSe is a variant caller designed for use with libraries prepared and sequenced using semi-degenerate barcoded adapters. These barcodes allow PCR and optical duplicates which were derived from the same starting molecule to be flagged and merged into a single consensus sequence. The addition of a strand-specific tag also allows molecules which are derived from different parental strands to be flagged, allowing variants at extremely low frequency (VAF~=0.1%) to be called confidently.

Additional Commands